How to Run an SDS-PAGE gel
4:44
YouTubelabtricks
How to Run an SDS-PAGE gel
Okay now that you know how to cast an SDS-PAGE gel, how exactly do you use it? Here, Dan shows you how to set up your apparatus to run SDS-PAGE. WAIT!!! Don't have a gel? Watch this video to learn how to make it: http://www.youtube.com/watch?v=EDi_n_0NiF4 Some important points to remember: - short glass plate faces inwards - remove the comb ...
405.5K viewsJan 24, 2010
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SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.
SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.
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